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Objective:To investigate the clinical effect of ultrasonic root canal intermittent irrigation in endodontic vascular reconstruction.Methods:32 young permanent teeth with pulp and periapical lesions were randomly divided into two groups ( n=32): experimental group was treated with ultrasonic root canal intermittent irrigation and the control group was rinsed with sodium hypochlorite root canal. The changes of periapical lesions and root development were evaluated by preoperative and postoperative clinical symptom observation and radiograph examination, and the clinical effect of endodontic vascular reconstruction was discussed. The time of inflammation control was discussed by analyzing the starting point from the first diagnosis to the date of mineral trioxide aggregate (MTA) coverage. Results:The clinical symptoms of the 16 affected teeth in the experimental group and the control group disappeared, the periapical lesions were eliminated, and the root continued to develop. In the experimental group, root formation was observed in 13 of the affected teeth, with a success rate of 81.25% and a reduction rate of 18.75%. In the control group, root formation was observed in 10 of the affected teeth, with a success rate of 62.5% and a reduction rate of 37.5%. There was no difference in the effective rate between the experimental group and the control group ( P>0.05). The control time of inflammation was (28.44±5.98)days in the experimental group and (34.13±7.17)days in the control group, with statistically significant differences ( P<0.05). Conclusions:Ultrasonic root canal intermittent swabbing applied to pulp revascularization can achieve good clinical effect and shorten the time of inflammation control.
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Objective To evaluate the effects of hypoxia inducible factor-1 alpha ( HIF-1α) and connective tissue growth factor (CTGF) proteins in the pathogenesis of oral submucous fibrosis (OSF). Methods Expressions of HIF-1αand CTGF proteins in 30 cases of OSF, and 5 cases of normale control o-ral mucosa tissue were studied with streptomyces peroxidase ( SP) immunohistochemistry with the HIF-1αand CTGF rabbit anti-human polyclonal antibodies.Results The negative expressions of HIF-1αand CT-GF proteins were found in normal control and significant increase was found in OSF ( P <0.05).Conclu-sions HIF-1αand CTGF proteins have positive expression in OSF, both of them may act as an important role in pathogenesis of OSF.
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Objective To understand oral submucous fibrous (OSF) because the hair cause of disease and the quantitative corresponding measures,do a good job in OSF prevention and control work.Methods The quantitative cluster sampling,according to the diagnostic criteria of the development of the Pindborg,yuhu in Xiangtan city of different types of 57 units of 11 046 people to chew areca cause OSF epidemiological investigation.Results OSF 335 diagnosis example,the prevalence rate of 30.33‰,4 cases were oral cancer,oral cancer coexist rate was 11.94‰; All OSF patients had a history of betel nut,no chewing betel nut (containing the cigarette,wine,and spicy aficionados) were not found in patients with OSF; Of OSF prevalence in the chewing hobby was no differences in sex and age in the crowd; OSF prevalence of high and low with length of fixed number of year of average chewing betel nut dose and chewing betel nuts were closely related(r =0.28828,P ＜ 0.01) ; OSF prevalence was different from eating betel nut additive that had a very significant difference.Different hobbies compatibility with standardized test,7 incidence group had 6 group without significant difference,but people only eat chili can (in the control group,1 329) and outsiders no OSF patients (control group,698).Conclusions Survey results confirm that chewing betel nut is the main factor of Xiangtan people of OSF,and OSF carcinoma prevalence is lower than abroad.
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Objective:To evaluate the effects of HIF-1αprotein in the pathogenesis of oral submucous fibrosis(OSF)tissue.Meth-ods:HIF-1αprotein expression in 30 cases of OSF,5 cases of normale control oral mucosa tissue was studied by SP immunohisto-chemistry with HIF-1αrabbit anti-human polyclonal antibody.Results:The negative expression of HIF-1αwas found in normal oral mucoa tissue and possitive in early,intermediate and advanced stage of OSF(P<0.05 ).Conclusion:HIF-1αprotein may play an importent role in the pathogenesis of OSF.
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<p><b>OBJECTIVE</b>To investigate the expression of DNAX-activating protein 12 (DAP12) and tartrate-resistant acid phosphatase (TRAP) in mouse monocyte RAW264.7 subjected to compressive stress.</p><p><b>METHODS</b>Mouse monocyte RAW264.7 was subjected to four-point bending system. The expression of DAP12 and TRAP mRNA were examined by reverse transcription-polymerase chain reaction (RT-PCR) and the expression of DAP12 protein by Western Blotting after 0, 3, 6 and 12h's compressive stress.</p><p><b>RESULTS</b>After RAW264.7 cells were cultured by osteoclast cell culture fluid, the amount of the cells increased, and the volume enlarged, and the number of nuclei per osteoclast increased in vitro. The expression of DAP12 and TRAP mRNA and DAP12 protein of RAW264.7 cells subjected to compressive stress increased along with the time (P < 0.05).</p><p><b>CONCLUSION</b>The mouse monocyte RAW264.7 can differentiate into osteoclast in vitro, and high expression of DAP12 exists in the process of osteoclast differentiation.</p>
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Animals , Mice , Cell Culture Techniques , Cell Differentiation , Cell Line , Monocytes , Osteoclasts , RNA, MessengerABSTRACT
Objective To investigate the effects of rotating pulsed magnetic field on platelet derived growth factor a (PDGF-A) expression in periodontal tissue during tooth movement. Methods 30 white rabbits were random divided into 6 groups with 5 rabbits each group,including groups of 1,3,5,7,14 and 21 days. Under anesthesia condition by 2% pentobarbital sodium,the stainless coil springs were fixed between the first maxillary molar and the incisor producing the force of 80g. The experimental group was treated by rotating pulsed magnetic field and the force, the control group was only treated by t he force. The expression of PDGF-A was half-quantitatively investigated through immunohistochemical analysis. Results The expression of PDGF-AA in the experimental group enhanced apparently compared with that in the control group. There were significant differences among the 5,7, and 14day groups (5. 28 ± 0. 14 vs 2. 03 ±0. 18,7.63±0.27 vs 2. 84 ±0. 12,3.52 ±0. 16 vs 1.65 ±0.03;8.10±0.13 vs 4. 30 ±0. 21,13. 27 ±0. 31 vs 6.47 ± 0.15,5.66 ± 0.22 vs 3. 15 ± 0. 27, P ＜ 0. 05). The expression of PDGF-AA of 7 day group was higher than that of other groups. Conclusion Rotating pulsed magnetic field promotes the expression of PDGF-A in the periodontal tissue and alveolar bone remodeling.
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<p><b>OBJECTIVE</b>To investigate the effects of cyclosporin A (CSA) on the level of intercellular adhesion molecule-1 (ICAM-1) expressed by oral fibroblasts (FB).</p><p><b>METHODS</b>The fibroblasts were obtained from normal buccal mucosa (NM-FB) and cultured in vitro. Then the levels of ICAM-1 expressed by fibroblasts incubated with or without CSA in the presence of 10% fetal calf serum for 48 hours at 37 degrees C in 5% CO(2) and air were monitored by using cell-based ELISA for ICAM-1.</p><p><b>RESULTS</b>We demonstrated that NM-FB had ICAM-1 expression (A = 0.324 +/- 0.030), and after 48 hours incubated with CSA the ICAM-1 levels expressed by oral buccal mucosa fibroblasts were dose-dependently decreased.</p><p><b>CONCLUSIONS</b>CSA reduces the levels of ICAM-1 expressed by oral mucosal FB, and may be useful in the treatment of some oral mucosal disorders.</p>
Subject(s)
Humans , Cells, Cultured , Cyclosporine , Pharmacology , Dose-Response Relationship, Drug , Enzyme-Linked Immunosorbent Assay , Fibroblasts , Cell Biology , Metabolism , Immunosuppressive Agents , Pharmacology , Intercellular Adhesion Molecule-1 , Mouth Mucosa , Cell Biology , MetabolismABSTRACT
Objective:In order to elucidate the derivation and differentiation mechanisms for derivation of myofibroblasts in oral submucous fibrosis.Methods:Oral keratinocytes and fibroblasts were isolated and cultured,and a co-cultur system composed of the keratinocytes and fibroblasts was established in vitro.Expressions of ?-SMA protein and mRNA in fibroblasts were detected by immunohistochemistry and RT-PCR. The level of TGF-?1 in conditioned medium were evaluated by ELISA.Results:The expressions of ?-SMA mRNA and protein in fibroblasts co-cultured with keratinocytes preprocessed by arecoline was higher than that in fibroblasts co-cultured with keratinocytes and without preprocessed by arecoline. The level of TGF-?1 in conditioned medium of fibroblasts-keratinocytes preprocessed by arecoline co-cultures was higher than those of fibroblasts cultured alone.Conclusion:The derivation of myofibroblast from FB can be promoted by keratinocytes preprocessed by arecoline and TGF-?1 may play a role in the process.
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Objective:To investigate the effects of arecoline on the expression of human telomerase reverse transcriptase(hTERT)mRNA and protein in cultured normal human oral mucosa keratinocytes(KCs),and then to investigate the role of hTERT in carcinogenesis of oral submucous fibrosis(OSF).Methods:Normal human oral mucosa KCs were cultured in vitro,and in experiment group,KCs was divided into 0.03,0.06,0.09 g/L arecoline group,and 0.0 g/L arecoline group served as the control.The hTERT mRNA and protein expression of KC was examined by reverse transcription polymerase chain reaction(RT-PCR)and Western blotting.Results:Arecoline induced the hTERT mRNA and protein expression of KC in a dose dependent manner.hTERT mRNA and protein expression of KC were increased by 0.03,0.06 and 0.09 g/L arecoline,when compared with the control group(P
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Objective:To investigate the role of human telomerase reverse transcriptase(hTERT) in carcinogenesis of oral submucous fibrosis(OSF).Methods:The expression of hTERT was examined by immunohistochemical SP method in 10 cases of normal oral mucosa, 46 cases of OSF,14 of carcinogenesis of OSF and 10 of adjacent non-cancerous OSF tissue. Results:The expression of hTERT was negative in normal oral muscosal epithelium.The frequency of hTERT positive expression was higher in carcinogenesis lesions of OSF(12/14) than those in OSF(7/46)(P
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Objective:To explore the effects of myofibroblast in the pathogenesis of oral submucous fibrosis(OSF).Methods:The expressions of ?-SMA in the tissues of OSF and normal control were detected by immunohistochemistry. Fibroblasts were isolated from oral mucosa of OSF patients and healthy controls and were cultured under stimulation by arecoline.The expressions of ?-SMA in OSF fibroblasts and normal fibroblast were detected by immunocellchemistry and RT-PCR.Results:(1)No positive expression was found in normal tissues except in the blood vessel,but in OSF tissue,?-SMA positive expression was found in many spindle cells in the oral submucousal layer.(2)Few fibroblasts in vitro from normal controls were ?-SMA positive.(3)Most of the second passage of OSF fibroblasts were ?-SMA positive(85.80%?3.56%). (4)The expression of ?-SMA in fibroblasts could not be increased by the stimulation of arecoline.Conclusion:Myofibroblasts may play an important role in the pathogenesis of OSF,and the appearrence of myofibroblasts in OSF may not be directly induced by the stimulation of arecoline.
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Objective:Dexamethasone (DEX) has been proved to be an effective therapy for a variety of oral mucosal disorders, especially for oral submucous fibrosis(OSF) .The mechanisms behind its therapeutic effects are not known but have largely been ascribed to its anti proliferation and immunosuppressive effects. The recruitment, extravagation and retention of leucocytes which may play an important role in the development of oral mucosal disorders depends in part on the interaction of LFA 1 with its ligand intercellular adhension molecules(ICAM 1).The study aimed to investigate the effects of DEX on proliferation and expression of ICAM 1 by human oral fibroblasts (FB).Methods:The fibroblasts were obtained from normal buccal mucosa (NM FB) and OSF buccal mucosa (OSF FB) and cultured in vitro. Then the cell proliferation of fibroblasts incubated with or without DEX in the presence of 10%fetal calf serum for 48 hours at 37℃ in 5%CO 2 and air were monitored by use of thiazolyl blue (MTT) assay and the level of ICAM 1 expressed by fibroblasts were monitored by using cell based ELSA for ICAM 1.Results:OSF FB had an increased proliferation compared to NM FB and DEX inhibited fibroblast proliferation in a concentration dependent manner; OSF FB produced ICAM 1 at high levels and DEX decreased ICAM 1 expression levels on both cell types.Conclusion:DEX can directly inhibit human oral fibroblasts proliferation and reduces the levels of ICAM 1 expressed by oral mucosal FB, and may be useful in the treatment of some oral mucosal disorders.
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Objective:To evaluate the effects of insulin-like growth factor 1(IGF-1) in the pathogenesis of oral submucous fibrosis(OSF).Methods:IGF-1 mRNA in 27 OSF tissues and 9 normal tissues were detected by the in situ hybridization technique.Results:There were expression of IGF-1 mRNA in keratinocytes of all the 8 cases of early stage OSF and 7 of the 19 cases of middle-late stage OSF.No expression of IGF-1 mRNA was detected in keratinocytes of nomal tissue.There were no significant difference between OSF tissues and normal tissues in the expression of IGF-1 mRNA in fibroblasts.IGF-1 mRNA expression in endothelial cells were found in 5 cases of middle-late stage.Conclusion:IGF-1 might be secreted by keratinocytes and may play important role in the pathogenesis of OSF.